Methods

As diverse as the disciplines to which our members belong (Physiology, Medical Biochemistry & Molecular Biology, Pharmacology & Toxicology, Physics, Biophysics, Pharmaceutical Biology, Structural Biology, Cell Biology, Clinical and Theroretical Medicine, Dentistry, Biology, Life Sciences, Computer Science, Pharmaceutical and Medicinal Chemistry), as varied are the methods used. Various primary human cell types, Yeast and transgenic mice are used as model systems. A selection of the technologies used by our member labs is given below.

 

 

Microscopy & Electrophysiology


  • Atomic force microscopy
  • Confocal laser-scanning microscopy
  • Ellipsometry and light microscopy
  • Electron microscopy
  • FACS (=fluorescence-activated cell scanning)
  • Fast video imaging
  • High-resolution live cell and tissue imaging
  • Membrane Potential Imaging
  • Structured illumination microscopy
  • Total internal reflection fluorescence microscopy
  • Two-photon imaging
  • X-ray diffractometry
  • Cryo-Electron Microscopy
  • Nuclear magnetic resonance (NMR) spectroscopy
  • Electrical measurements of exocytotic fusion pores
  • Photolysic ‘uncaging’ of intracellular calcium
  • Patch clamp techniques: (Whole-cell, Voltage-clamp, Current-clamp, Single-channel, Perforated patch, Loose-patch, Cell-attached)
  • Field potential recordings

Biochemistry / Cell & Molecular Biology


  • Biochemical proximity labeling methods
  • Generation of antibodies
  • Immunohistochemistry
  • Mass spectrometry
  • FACS (=fluorescence-activated cell scanning)
  • Fast video imaging
  • High-resolution live cell and tissue imaging
  • Membrane Potential Imaging
  • Two-photon imaging
  • Non-contact cell or sarcomere length measurement
  • Human tissue culture
  • Proteomics
  • Western blot
  • Plasmon Resonance Spectroscopy
  • Adeno-associated virus mediated gene transfer in vivo
  • DNA/RNA Microarrays
  • miRNAs
  • Northern/Southern Blot
  • Quantitative (real-time) polymerase chain reaction (q-PCR)
  • Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
  • Systematic mutagenesis

Pharmacology


  • Drug therapy
  • Pharmacovigilance
  • Analytical and preparative cell sorting
  • Protein analytics
  • Laboratory for LTRT and SPF experimental laboratory animal husbandry